何玉财 简介

发布时间:2017-05-12浏览次数:105

何玉财 博士/副教授/硕导

Email: heyucai2001@163.com; yucaihe@cczu.edu.cn

  

近几年主持的项目:

国家自然科学基金:

环硫酸酯对映选择性微生物水解反应特性及催化机制研究(No. 211020112012-2014

省市基金:

(1江苏省自然科学基金:(No. BK20141172) 2015-2017

(2) 常州市社会发展项目:(No. CE20115051) 2011-2012

常州大学博士基金:

腈水合酶法合成西司他汀的重要中间体S-(+)-2,2-二甲基环丙甲酰胺研究(No. ZMF10020077) 2010.7-2012.6

开放课题:

(1) 发酵工程省部共建教育部重点实验室(湖北工业大学)开放课题:高活性甲壳素酶的选育及在甲壳素生物转化中的应用2010-2012

(2)  生物反应器工程国家重点实验室开放课题:酶法水解羟基腈生产高纯度手性羟基羧酸的应用基础研究(No 008004) 2008-2010

(3) 生物反应器工程国家重点实验室开放课题:基于高效原位酶解纤维素体系固定化纤维素酶催化机制及其应用研究2011-2013

  

本课题组成员:
 2012
届硕士研究生:周琼(联合培养);

2013届硕士研究生:夏东琴(联合培养);

2014届硕士研究生:杨振兴,龚磊,潘雪鹤(联合培养);

2013级硕士研究生:陶志成,张丹平;

2014级硕士研究生:刘峰,丁云;

2015级硕士研究生:种刚刚;

RESEARCHEXPERIENCE

主要从事生物催化与转化、生物降解、生物质转化研究。

  

Biomassand Bioenergy

Bioconversion of cellulosic materials tofuel ethanol and other value-added products and chemicals

Specific Research Emphasis: a).Pretreatment of cellulosic materials and related reaction system design andkinetics study; b). Investigation of the synergetic effects between cellulaseand hemicellulase during enzymatic hydrolysis and its mechanics; c).Fermentation of sugars for production of bioethanol and other fuels; d). Investigationof the relationship between pretreatment and enzymatic hydrolysis and methodsto improve the overall cellulose and hemicelluloses conversion; e). Screeningof cellulase-producing strain and its fermentation optimization.

Biocatalysis

Biosynthesis of optically pure chiralcompounds or other important intermediates with extremely high values forpharmaceuticals and agrochemicals

Specific Research Emphasis: a).Screening and development of a lot of highly enantioselelctive biocatalysts bysubstrate-oriented screening, such as nitrilases, esterases and oxidoreductases;b). For production of highly value-added chiral synthons or otherintermediates, many efficient biotransformation processes, either by manipulatingthe medium composition (e.g., addition of organic cosolvents, metal ions,inducers, or surfactants like Tween-80) and the reaction conditions, or by optimizingthe bioreactor configuration or its operation mode, resulting in significant enhancementof selectivity and productivity; c). Immobilization technology is used forenhancing operation stability; d). Clone and overexpression of nitrilase and reductase.

  

SELECTEDPUBLICATION:

1. Effective pretreatment of diluteNaOH-soaked chestnut shell with glycerol-HClO4-water media:Structural characterization, enzymatic saccharification, and ethanolfermentation. Bioprocess and Biosystems EngineeringDOI:10.1007/s00449-015-1535-z. (通讯作者)

2. Enzymatic insitu saccharification of chestnut shell with high ionic liquid-tolerantcellulases from Galactomycessp.CCZU11-1 in a biocompatible ionic liquid-cellulase mediaBioresourceTechnology 2016, 201: 133-139. (通讯作者)

3.Effective enzymatic saccharification of dilute NaOH extraction of chestnutshell pretreated by acidified aqueous ethylene glycol media. IndustrialCrops and Products 2016, 81: 129-138. (通讯作者)

4.Improving enzymatic hydrolysis of corn stover pretreated by ethylene glycol-perchloricacid-water mixture.Applied Biochemistry and Biotechnology,2015, 175: 1306-1317. (通讯作者)

5. Biotransformationof 1,3-propanediol cyclic sulfate and its derivatives to diols bytoluene-permeabilized cells ofBacillus sp.CCZU11-1.Applied Biochemistry and Biotechnology, 2015, 175(5): 2647-2658.(通讯作者)

6. Enhancementof enzymatic saccharification of corn stover with sequential Fentonpretreatment and dilute NaOH extractionBioresource Technology 2015,193: 324-330. (通讯作者)

7. Removal of Cu2+from aqueous solutions by the novel modified bagasse pulp cellulose: Kinetics,isotherm and mechanismCarbohydrate Polymers 2015, 129:115-126. (通讯作者)

8.Significantlyimproving enzymatic saccharification of high crystallinity index’s corn stoverby combining ionic liquid [Bmim]Cl–HCl–water media with dilute NaOHpretreatmentBioresource Technology 2015, 189: 421-425.(通讯作者)

9. Effectivebiosynthesis of ethyl (R)-4-chloro-3-hydroxybutanoateby supplementation of L-glutamine, D-xylose and β-cyclodextrin in n-butylacetate–water mediaJournal of Biotechnology 2015,203:62-67. (通讯作者)

10. Biosynthesisof ethyl (S)-4-chloro-3-hydroxybutanoatewith an NADH-dependent reductase (ClCR) discovered by genome data mining usinga modified colorimetric screening strategy. Bioengineered 2015, 6(3): 170-174. (通讯作者)

11. Improved biosynthesis of ethyl (S)-4-chloro-3-hydroxybutanoate by addingL-glutamine plus glycine instead ofNAD+ in β-cyclodextrin−water system. Bioresource technology 2015,182: 98-102. (通讯作者)

12. Biotransformation of1,3-propanediol cyclic sulfate and its derivatives to diols by Rhodococcus sp.Biotechnology Letters,2015, 37: 183-188. (通讯作者)

13. Biosynthesis of ethyl (S)-4-chloro-3-hydroxybutanoate byNADH-dependent reductase from E. coli CCZU-Y10discovered by genome data mining using mannitol as cosubstrate. AppliedBiochemistry and Biotechnology, 2014, 173: 2042-2053. (通讯作者)

14. Biosynthesis of benzoylformic acidfrom benzoyl cyanide with a new bacterial isolate of Brevibacterium sp. CCZU12-1. Applied Biochemistry and Biotechnology,2014, 72(6): 3223-3233. (通讯作者)

15. Bioproduction of glycolic acidfrom glycolonitrile with a new bacterial isolate of Alcaligenes sp. ECU0401.Applied Biochemistry and Biotechnology,2010, 160: 1428-1440. (通讯作者)

16. Waste biogas residue from cassava dregsas carbon source to produce Galactomycessp. Cczu11-1 cellulase and its enzymatic saccharification. Applied Biochemistry andBiotechnology, 2014, 173: 894-903. (通讯作者)

17. Biosynthesis of terephthalic acid,isophthalic acid and their derivatives from the corresponding dinitriles by tetrachloroterephthalonitrile-inducedRhodococcus sp. Biotechnology Letters,2014, 36: 341-347. (通讯作者)

18. Discovery of a reductase-producingstrain recombinant E. coli CCZU-A13using colorimetric screening and its whole cell-catalyzed biosynthesis of ethyl(R)-4-chloro-3-hydroxybutanoate. BioresourceTechnology, 2014, 172: 342-348. (通讯作者)

19. Highly efficient synthesis ofethyl (S)-4-chloro-3-hydroxybutanoateand its derivatives by a robust NADH-dependent reductase from E. coli CCZU-K14. Bioresource Technology, 2014,161: 461-464. (通讯作者)

20. Highly enantioselective oxidationof phenyl methyl sulfide and its derivatives into optically pure (S)-sulfoxides with Rhodococcus sp. CCZU10-1 in an n-octane-water biphasic system. AppliedMicrobiology and Biotechnology, 2013, 97: 10329-10337. (通讯作者)

21. Highly enantioselective oxidationof racemic phenyl-1,2-ethanediol to optically pure (R)-(-)-mandelic acid by a newly isolated Brevibacterium lutescens CCZU12-1. Applied Microbiology andBiotechnology, 2013, 97: 7185-7194. (通讯作者)

22. Enzymatic saccharification ofsugarcane baggage by N-methylmorpholine-N-oxide-tolerant cellulase from a newlyisolated Galactomyces sp. CCZU11-1. BioresourceTechnology, 2013, 135: 18-22. (通讯作者)

23. Biosynthesis of benzoylformic acidfrom benzoyl cyanide by a newly isolated Rhodococcussp. CCZU10-1 in toluene-water biphasic system. Bioresource Technology,2012, 115: 88-95. (通讯作者)

24. A high-throughput screeningstrategy for nitrile-hydrolyzing enzymes based on ferric hydroxamatespectrophotometry. Applied Microbiology and Biotechnology, 2011, 89: 817-823. (通讯作者)

25. Biocatalytic synthesis of (R)-(-)-mandelic acid from racemicmandelonitrile by cetyl-trimethylammoniumbromide-permeabilized cells of Alcaligenes faecalis ECU0401.Journal of Industrial Microbiology and Biotechnology, 2010, 377:741-750. (第一作者)

26. Improving enzymatic hydrolysis ofwheat straw using ionic liquid 1-ethyl-3-methyl imidazolium diethyl phosphatepretreatment. Bioresource Technology, 2010, 100(14): 3570-3575. (第二作者)

27. A Bronsted acidic ionic liquid asan efficient and environmental benign catalyst for biodiesel synthesis fromfree fatty acids and achohols. Bioresource Technology, 2009,100(19): 4368-4373. (第三作者)